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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive <t>GnRH</t> <t>stimulation</t> test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.
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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive <t>GnRH</t> <t>stimulation</t> test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.
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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive <t>GnRH</t> <t>stimulation</t> test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.
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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive <t>GnRH</t> <t>stimulation</t> test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.
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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive <t>GnRH</t> <t>stimulation</t> test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.
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Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive GnRH stimulation test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.

Journal: Translational Pediatrics

Article Title: Diagnostic value of serum inhibin B, anti-Müllerian hormone, and estradiol for central precocious puberty and early-fast puberty in girls: a cross-sectional study

doi: 10.21037/tp-2026-1-0037

Figure Lengend Snippet: Distribution of key biomarkers across study groups. Boxplots showing serum levels of (A) AMH, (B) INHB, (C) basal FSH, (D) basal LH, (E) E 2 , (F) UV, and (G) OV in EFP, CPP, PT, and healthy controls. One outlier in (D) (basal LH >2 IU/L within the CPP group) was clinically verified; this patient had borderline basal gonadotropin elevation at enrollment, met all other CPP inclusion criteria including a positive GnRH stimulation test, and was retained in the analysis. AMH, anti-Müllerian hormone; CPP, central precocious puberty; E 2 , estradiol; EFP, early and fast puberty; FSH, follicle-stimulating hormone; GnRH, gonadotropin-releasing hormone; INHB, inhibin B; LH, luteinizing hormone; OV, ovarian volume; PT, premature thelarche; UV, uterine volume.

Article Snippet: For CPP and EFP participants, a standard GnRH stimulation test was conducted: 2.5 μg/kg (max 100 μg) synthetic GnRH (Ferring Pharmaceuticals) was administered intravenously, with serial LH/FSH measurements at 0, 30, 60, and 90 minutes post-injection, Gonadotropin responses were measured on the ADVIA Centaur XP platform, and HPGA activation was defined as a peak LH ≥5.0 IU/L and a peak LH/FSH ratio ≥0.6, consistent with current consensus recommendations.

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